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Kinetic Studies on Endo-β-galactosidase by a Novel Colorimetric Assay and Synthesis of Poly-N-acetyllactosamines Using Its Transglycosylation Activity
endo-β-galactosidase enzyme assay poly-N-acetyllactosamine kinetics transglycosylation
2008/4/20
Novel chromogenic substrates for endo-β-galactosidase were designed on the basis of the structural features of keratan sulfate. Galβ1,4GlcNAcβ1,3Galβ1,4GlcNAcβ-pNP (2), which consists of two repeating...
Transglycosylation of Asparagine-linked Complex-type Oligosaccharides from Glycoproteins by Endo-β-N-acetylglucosaminidase HS
endo-β-N-acetylglucosaminidase HS endo HS asparagine-linked oligosaccharide glycoprotein transglycosylation
2008/4/19
Endo-β-N-acetylglucosaminidases hydrolyze N,N´-diacetylchitobiose linkages of asparagine-linked oligosaccharides. They can also cleave the linkage with suitable agents having hydroxyl groups and...
Transglycosylation of Mogroside V, a Triterpene Glycoside in Siraitia grosvenori, by Cyclodextrin Glucanotransferase and Improvement of the Qualities of Sweetness
mogroside V transglycosylation cyclodextrin glucanotransferase sweetness quality of sweetness
2008/4/19
Mogroside V (MV), a main sweet triterpene glycoside in the extract of the fruit of Luo-han-guo (Siraitia grosvenori Swingle), was transglycosylated by cyclodextrin glucanotransferases (CGTases) using ...
Transglycosylation Reaction and Acceptor Specificity of Exo- and Endo-type Cellulases
cellulase transglycosylation acceptor specificity
2008/4/19
The possibility of transglycosylation and acceptor specificity of cellulases, CBH I, CBH II, EG II (Trichoderma reesei), Ex-1 (Irpex lacteus) and Exo-A (Aspergillus niger) was investigated by using ce...