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Studies on Low-cost Substrates and Other Cultural Conditions for Optimal Amylase Yield from Aspergillus oryzae-SR2
Cultural Conditions Low-cost Substrates
2016/6/2
The need for starch-converting enzymes in various industries has led to enormous interest in their production at a cheap rate. The present study was focused on the examination of various inexpensive s...
Analysis of the Promoter Activity of the Taka-Amylase Gene and the Phosphoglycerate Kinase Gene in a Shoyu-koji Mold Aspergillus oryzae KBN616
Aspergillus oryzae phosphoglycerate kinase promoter
2009/7/8
Promoter activity of the genes encoding Taka-amylase A and phosphoglycerate kinase of Aspergillus oryzae was analyzed using Escherichia coli β-glucuronidase as a reporter in a shoyu-koji mold A. oryza...
Synergy Between an α-L-Arabinofuranosidase from Aspergillus oryzae and an Endo-Arabinanase from Streptomyces coelicolor for Degradation of Arabinan
arabinan α-L-arabinofuranosidase glycoside hydrolase family 43
2009/6/18
An α-L-arabinofuranosidase gene of Aspergillus oryzae was expressed in Pichia pastoris. The re-combinant enzyme released L-arabinose from arabinose-containing polysaccharides such as lupin pectic gala...
Purification and Characterization of Glutamate Decarboxylase from Aspergillus oryzae
glutamate decarboxylase Aspergillus oryzae purification
2009/6/17
We purified glutamate decarboxylase (GAD) [EC4.1.1.15] from Aspergillus oryzae and characterized its biochemical and kinetic properties. GAD was purified by ammonium sulfate at 30–70% saturation and c...
Studies on Aspergillus oryzae Mutants for the Production of Single Cell Proteins from Deoiled Rice Bran
solid state fermentation mutants screening deoiled rice bran
2009/3/19
Ethyl methyl sulphonate was used to induce point mutation in Aspergillus oryzae (MTCC 1846). Incubation with ethyl methyl sulphonate for 1 h resulted in 98 % killing of spores. By screening the surviv...
Use of Potato Nitrogen Concentrate in the Production of a-Amylase by Aspergillus oryzae
a-amylase Aspergillus oryzae potato nitrogen concentrate (PNC)
2009/3/3
The influence of various nitrogen sources and media supplements on a-amylase (EC 3.2.1.1) formation by Aspergillus oryzae ATCC 1011 was investigated in shake flask experiments and batch fermentations....
Expression of Aspergillus oryzae Tannase in Pichia pastoris and Its Application in the Synthesis of Propyl Gallate in Organic Solvent
tannase Pichia pastoris recombinant protein production
2009/2/25
Gallic acid esters could be synthesized biologically by tannase in organic media, among which is propyl gallate, an antioxidant widely used as a food additive. Efficient intracellular expression of As...
Chemical and Microbiological Characteristics of Sardine Meal Fermented with Aspergillus oryzae IFO 4202
fermentation quality improvement bacterial flora
2010/11/22
Sardine moist meals were inoculated with Aspergillus oryzae IFO 4202 seed and fermented. Accompanying the growth of the fungus, the chemical composition of the meals significantly changed during incub...