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Searching for Genes of Lactococcus lactis subsp. lactis Encoding the Bacteriocin Nisin using DNA/DNA Hybridisation
Lactococcus lactis subsp .lactis screening nisin gene PCR probe DNA/DNA hybridisation
2014/3/10
n this work, PCR primers P8 and P9 were used for amplification of a 320 bp long PCR product specific to the nisin gene. The PCR product was labelled with digoxigenine during amplification and used as ...
Identification and characterisation of antimicrobial activity of nisin a produced by Lactococcus lactis subsp. lactis LL27
nisin A Lactococcus lactis sublethal injury heating freezing
2014/3/10
In this study, bacteriocin producing lactococcal strains were isolated from Turkish raw milk samples. Among these isolates, LL27 had the highest inhibition activity against Gram-positive indicator str...
Growth Associated Exopolysaccharide Expression in Lactococcus lactis subspecies cremoris Ropy352
ropy Lactococcus lactis exopolysaccharide
2008/4/1
A natural lactococcal isolate, Lactococcus lactis ssp. cremoris Ropy352, has been previously shown to express two phenotypically distinct exopolysaccharides (ropy and mucoid). This natural isolate was...
Membrane Receptor for Prolate Phages is Not Required for Infection of Lactococcus lactis by Small or Large Isometric Phages
Lactococcus lactis electroporation allele replacement bacteriophage receptor
2008/3/28
Lactococcus lactis contains a chromosomal gene (pip) for a membrane protein that serves as a receptor for the prolate bacteriophage c2 and other phages of the c2 species. A mutated allele of this rece...
High Titer, Phage-Neutralizing Antibodies in Bovine Colostrum that Prevent Lytic Infection of Lactococcus lactis in Fermentations of Phage-contaminated Milk
Lactococcus lactis phage lactococcal bacteriophage colostrum • antibodies
2008/3/28
Antibodies against six phages of Lactococcus lactis were produced in six bovine colostra. Each colostrum neutralized its homologous phage. In addition, each colostrum neutralized a different phage fro...
Peptide Accumulation and Bitterness in Cheddar Cheese Made Using Single-Strain Lactococcus lactis Starters with Distinct Proteinase Specificities
Lactococcus lactis proteinase cheese proteolysis bitterness
2008/3/28
This study investigated peptide accumulation and bitterness in reduced- and full-fat Cheddar cheeses that were manufactured with single-strain Lactococcus lactis starters that had distinct cell envelo...
Expression of Ropy and Mucoid Phenotypes in Lactococcus lactis
ropy phenotype mucoid phenotype Lactococcus lactis Lon protease
2008/3/27
Strains of Lactococcus lactis ssp. lactis and Lactococcus lactis ssp. cremoris were cultured under aerobic and anaerobic conditions on plates of whey agar, Elliker agar, and M17L agar at 15, 20, and 3...
Associative Growth of Lactococcus lactis and Leuconostoc mesenteroides Strains in Milk
Leuconostoc mesenteroides Lactococcus lactis mixed-strain culture milk
2008/3/27
The associative growth of a proteinase-positive strain of Lactococcus lactis ssp. lactis CNRZ 1076 and Leuconostoc mesenteroides ssp. mesenteroides UM10 in reconstituted skim milk was studied in detai...
Bacteriophage Resistance in Lactococcus lactis Engineered by Replacement of a Gene for a Bacteriophage Receptor
phage resistance bacteriophage pip gene replacement
2008/3/27
The objective of this study was to construct a food-grade, phage-resistant strain of Lactococcus lactis by replacing a specific chromosomal gene with an allele that had been mutated in vitro. Lactococ...
Effect of Citrate on Production of Diacetyl and Acetoin by Lactococcus lactis ssp. lactis CNRZ 483 Cultivated in the Presence of Oxygen
Lactococcus lactis ssp lactis CNRZ 483 citrate diacetyl acetoin
2008/3/27
The effect was studied of trisodium citrate addition on the growth and formation of diacetyl and acetoin by Lactococcus lactis ssp. lactis CNRZ 483 in a whey-based medium under an oxygen pressure of 2...
Removal of Bitterness from the Bitter Peptides Extracted from Cheddar Cheese with Peptidases from Lactococcus lactis ssp. cremoris SK11
bitterness removal peptidase Lactococcus lactis ssp. cremoris SK11
2008/3/26
Intracellular peptidases were extracted from the cell lysate of Lactococcus lactis ssp. cremoris SK11 that had been grown in milk. Peptidase activities were determined using 12 synthetic derivatives o...
Mixed Cultures of Proteinase-Positive and Proteinase-Negative Strains of Lactococcus lactis in Milk
Lactococcus lactis proteinase mixed culture milk
2008/3/26
Twenty-four mixed cultures of nonisogenic proteinase-positive and proteinase-negative strains of Lactococcus lactis (initial ratio, 1:9) were grown in milk. The proteinase-negative strains were system...
Improvement of Diacetyl Production by Lactococcus lactis ssp. lactis CNRZ 483 Through Oxygen Control
Lactococcus lactis ssp. lactis diacetyl oxygen
2008/3/26
This study illustrated the effect of controlled oxygenation on diacetyl production by citrate-utilizing Lactococcus lactis ssp. lactis CNRZ 483. Effects of soluble oxygen concentrations at 21 and 50% ...
ß-Casomorphins: Analysis in Cheese and Susceptibility to Proteolytic Enzymes from Lactococcus lactis ssp. cremoris
ß beta -casomorphins cheese proteolysis Xprolyl dipeptidyl aminopeptidase
2008/3/25
Commercial cheese products were surveyed for ß-casomorphin peptides. Two extraction methods were compared: 1) water and 2) chloroform and methanol. Peptide profiles were determined using reverse...
Purification and Characterization of a Cysteine Proteinase with Low Activation Energy from Lactococcus lactis ssp. lactis IAM 1198
proteinase Lactococcus lactis ssp. lactis
2008/3/23
A caseolytic proteinase with low activation energy was purified to homogeneity by conventional chromatographic techniques from Lactococcus lactis ssp. lactis IAM 1198. The molecular weight of the enzy...