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In vitro Binding Capacity of Cholesterol and Bile Salts by Partially Depolymerized Chitosans
Cholesterol and Bile Salts Partially Depolymerized Chitosans
2016/5/31
Orthogonal design was used to optimize the binding capacity of Sodium Cholate (SC) and Sodium Deoxycholate (SD) by nine partially depolymerized chitosans obtained from a native chitosan using sodium n...
The Effect of Bovine Somatotropin and Diet on Somatotropin Binding Sites in Hepatic Tissue of Lactating Dairy Cows
binding sites dairy cows liver somatotropin
2008/7/23
In the lactating cow, galactopoiesis is stimulated by treatment with recombinant bovine somatotropin (bST) and by an improved plane of nutrition. The present study determined the interaction between t...
Functional Improvement of Xylanase by Introducing Mutated Xylan-binding Domain
xylanase, xylan-binding domain, phage display, directed evolution
2008/4/20
Alkaliphilic Bacillus sp. strain 41M-1 secretes a xylanase (termed xylanase J) that has an alkaline pH optimum. Xylanase J is a multidomain enzyme and consists of two functional domains: a glycoside h...
Identification and Manipulation of Subsite Structure and Starch Granule Binding Site in Plant α-Amylase
α-amylase subsite starch granule
2008/4/20
In germinating plant seeds, α-amylases degrade starch accumulated in seeds, and that requires two functions: catalysis itself and starch granule binding ability. All plant α-amylases belong to the α-a...
Determination and Analysis of the Starch Binding Domain of Streptococcus bovis 148 Raw-Starch-Hydrolyzing α-Amylase
Streptococcus bovis α-amylase linker starch binding domain CBM26
2008/4/20
We analyzed the functional structure of Streptococcus bovis 148 α-amylase (AmyA) to determine the starch binding domain (SBD) of this enzyme. Several derivative AmyAs and putative SBD segments were co...
Production of Aldonic Acids from Monosaccharides by Washed Cells of Burkholderia cepacia and their Calcium Binding Capability
aldonic acid Burkholderia cepacia microbial conversion oxidation calcium binding
2008/4/20
Microbial oxidation of monosaccharides and calcium binding capability of the resulting aldonic acids were investigated. When washed cells of a strain of Burkholderia cepacia were shaken with 15% (w/v)...
Engineering Thermus Maltogenic Amylase with Improved Thermostability: Probing the Role of the Conserved Calcium Binding Site in Cyclodextrin-degrading Enzymes
calcium binding site cyclodextrin-degrading enzymes mutagenesis thermostability Thermus maltogenic amylase
2008/4/20
Thermus maltogenic amylase (ThMA), one of the cyclodextrin (CD)-degrading enzymes, is expected to have a calcium-binding site (Ca2 site) based on multiple sequence alignments. In spite of the presumpt...
Substrate-Binding Mode of Bacterial Chitosanases
chitosanase, substrate binding, fluorescence, thermal unfolding, X-ray crystallography
2008/4/19
Mode of substrate-binding of chitosanases from Streptomyces sp. N174 (N174 chitosanase) and Bacillus circulans MH-K1 (MH-K1 chitosanase) was examined by site-directed mutagenesis and physicochemical t...
Viability of Lactobacillus gasseri and Its Cholesterol-Binding and Antimutagenic Activities During Subsequent Refrigerated Storage in Nonfermented Milk
viability sodium taurocholate deconjugation cholesterol-binding ability antimutagenicity
2008/3/31
The effect storage at 4°C on the viability of Lactobacillus gasseri and its sodium taurocholate-deconjugating and cholesterol-binding abilities as well as desmutagenic activity was investigated. Unfer...
New Binding Assay and Preparative Trial of Cell-Surface Lectin from Lactobacillus acidophilus Group Lactic Acid Bacteria
Lactobacillus acidophilus lectin rat colonic mucosa surface layer protein
2008/3/31
To select Lactobacillus acidophilus group bacteria as a probiotic yogurt starter, we designed a new screening method that measures the binding activity of surface layer protein to rat colonic mucin, w...
Binding of Lipophilic Nutrients to ß-Lactoglobulin Prepared by Bioselective Adsorption
binding lipophilic nutrients ß beta -lactoglobulin bioselective adsorption
2008/3/28
The binding of the lipophilic nutrients, retinal, vitamin D2, and retinyl palmitate by ß-lactoglobulin was measured by analysis of changes in the fluorescence of the tryptophanyl residue of &szl...
Bile Tolerance, Taurocholate Deconjugation, and Binding of Cholesterol by Lactobacillus gasseri Strains
bile tolerance sodium taurocholate deconjugation cholesterol-binding ability
2008/3/28
Bile tolerance, deconjugation of sodium taurocholate, and the cholesterol-binding ability of 28 strains of Lactobacillus gasseri were examined. There was significant variation among strains in growth ...
Preparation of Phosphopeptides Derived from s-Casein and ß-Casein Using Immobilized Glutamic Acid-Specific Endopeptidase and Characterization of Their Calcium Binding
casein phosphopeptides immobilized enzyme glutamic acid-specific endopeptidase calcium binding
2008/3/28
Phosphopeptides that were derived from s-CN or ß-CN were prepared with immobilized glutamic acid-specific endopeptidase, and their Ca2+ binding was characterized. s-Casein or ß-CN was hydr...
Calcium Binding of Phosphopeptides Derived from Hydrolysis of a s-Casein or ß-Casein Using Immobilized Trypsin
casein immobilized trypsin casein phosphopeptide calcium binding
2008/3/28
Calcium binding to casein phosphopeptides that were derived from s-CN or ß-CN was studied. Purified s-CN or ß-CN was prepared from fresh skim milk using an anion-exchange column. Peptides ...
Properties of a Heparin-binding Peptide Derived from Bovine Lactoferrin
lactoferrin heparin binding conformation
2008/3/28
A heparin-binding peptide was isolated from a proteolytic hydrolysate of bovine lactoferrin by affinity chromatography using an immobilized heparin column. Analysis of amino acid sequences at the N-te...