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Improvement of the Enzymatic Properties of Kojibiose Phosphorylase from Thermoanaerobacter brockii by Random Mutagenesis and Chimerization
kojibiose phosphorylase random mutagenesis chimerization Thermoanaerobacter brockii
2008/4/20
Random mutagenesis by error-prone PCR was introduced to kojibiose phosphorylase (KP; EC 2.4.1.230) from Thermoanaerobacter brockii ATCC35047. One thermostable mutant and two DP-mutants that were defin...
Isolation and Identification of Novel Tri- and Tetra-saccharides Synthesized by Thermoanaerobacter brockii Kojibiose Phosphorylase
Kojibiose phosphorylase oligosaccharide Palatinose NMR
2008/4/20
Novel tri- and tetra-saccharides were synthesized by glucosyltransfer from β-D-glucose 1-phosphate (β-D-G1P) to palatinose using Thermoanaerobacter brockii kojibiose phosphorylase. There saccharides w...