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The effect of the phenotypic combination of genetic variants κ-CN-Lg on the contents of technologically and nutritionally important components of milk as well as technological properties of milk was...
The aggregation rate of B-Lactoglobulin (BLG) was studied using turbidimetry and dynamic light scattering in the range 5.8at a fixed ionic strength of 4.5 mM, and in the range 4.5 - 500mM NACl...
Freshly fractionated β-lactoglobulin AB-Lg) from Cheddar whey was dispersed at pH 3.5, 7, and 9 buffers containing 20 mM EDTA, and circulated at 25°C through a closed loop containing a 200 nm pore ...
Many studies on the conjugates of proteins and saccharides (neoglycoproteins) have been carried out over the past 20 years to improve the function of proteins. However, most have been carried out by a...
Milk samples from 248 Pag ewes, belonging to 14 different flocks and located through the Pag Island (Croatia), were analyzed by isoelectrofocusing and PCR-RFLP. Two genetic variants (A and B) and thre...
β-Lactoglobulin-LG) was modified and conjugated to xylobiose using the Maillard reaction. The antioxidant activity of the Maillard reaction product, β-LG-xylobiose, was measured in vitro and compar...
The binding of the lipophilic nutrients, retinal, vitamin D2, and retinyl palmitate by ß-lactoglobulin was measured by analysis of changes in the fluorescence of the tryptophanyl residue of &szl...
Differential scanning calorimetry was used to study the thermal stability of bovine ß-LG as influenced by binding of palmitic acid or retinol. Maximum peak temperature and apparent enthalpy of d...
The binding of palmitate to ß-lactoglobulin at protein concentrations ranging from 1 to 200 µM was determined using an ultrafiltration method with [14C]palmitate. Fit of the data to theore...
The extent of the early stage of the Maillard-type reaction that impaired functional properties of whey proteins was evaluated by electrospray ionization mass spectrometry. Under conditions of mild he...
ß-Lactoglobulin was isolated directly from acidic whey by bioselective adsorption on N-retinyl-CeliteTM, yielding preparations of 96% purity. Interactions of these preparations with vitamin D2, ...
Binding of the retinoids, all-trans-retinol, all-trans-retinal, all-trans-retinyl acetate, and all-trans-retinoic acid, to ß-lactoglobulin (LG) (96% purity) that had been prepared by bioselectiv...
Western blotting of bovine ß-LG is a valid method to detect adulteration by pasteurized bovine milk, by UHT bovine milk, or by heat-denatured bovine whey proteins in cheeses made of milk from ot...
A method for the separation of the ß-LG variants A, B, and C was developed using free zone capillary electrophoresis. A separation buffer consisting of 50 mM 2-(N-morpholino)ethane sulfonic acid...
The susceptibility of ß-LG and sodium caseinate to proteolysis by pepsin and trypsin was investigated using SDS or urea-PAGE. The effects were studied of heat, urea, and 2-mercaptoethanol on pro...

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